|Label||The Take-No-Prisoners Jock-ette|
|Episode (s) Eliminated||Saving Private Leechball|
|Enemies||Alejandro, Anne Maria, Brick, Cameron, Chris, Chef, Courtney, Duncan, Ezekiel, Gwen, Heather, Lightning, Lindsay, Mike, Sam, Scott, Sierra, Zoey|
|First Appearance||"Heroes Vs. Villains"|
|Latest Appearance||"The Final Wreck-ening"|
|Voiced by||Laurie Elliot|
OverviewChef Hatchet, but is thrown out of the plane. She is shocked along with the other contestants when Chris pranks them with Ezekiel. On the beach, Jo is put into the Villainous Vultures team along with Heather, Scott, Lightning, Duncan, and Gwen. She complains that the Heroic Hamsters have an unfair numbers advantage over them, with seven team members to six, prompting Chris to put Alejandro (trapped in the Drama Machine) on the Vultures. On the way to the challenge, Jo and Heather begin to clash, after Heather pushes her out of the way and Jo calls her "old heather". During the challenge, Jo volunteers as the pusher for her team, and proposes an alliance to Lightning. After Duncan retrieves his key she is slightly suprised when he compliments her after she insults him with one of her "zingers". After Alejandro is revealed to be in the robot suit, Jo carries him because of the uselessness of his legs, to the hotel, where he unlocks it with the correcy key, winning the challenge for the vultures.
Heather into the crab pit in retaliation. Jo begins to grow annoyed with Lightning after he steals the statue piece that she finds, and after he hurls another piece at Jo's head, causing her to fall into the crab pit herself. Jo laughs at Sam after he gets stung by a jellyfish, along with the other vultures. When Lightning loses the challenge for the Vultures because he fails to count the correct number of pieces and knocks down the statue, Jo starts a petition to send him home. Her goal is acheived when Lightning is sent to the Flush of Shame at the marshmallow ceremony, although she is in the bottom two.Scott instead, earning a point for the Hamsters, causing her team to get angry at her. After Heather is shot, and Duncan and Gwen leave to take care of Mike, Jo goes to find Zoey, arming herself with two guns. She finds Sam and Courtney hiding in a cave, and manages to shoot at Sam but is beaten by Zoey, who wins the challenge for the Hamsters, and sending the Vultures to elimination for a second time. At the marshmallow ceremony, Jo's team votes her out because of her poor aim, and she is flushed down the flush of shame after Duncan and Courtney switch teams.
Jo ends up inside a tomb in Egypt, where she notices several scarabs with her as well. She is initially not afraid of them, but quickly notices that the insects start to surround her in large numbers. As Jo starts to scream in terror, the camera zooms out to the streets above her.
The Final Wreck-ening
She is in one of the balloons in The Final Wreck-ening, but her balloon is not popped, and floats away with the other un-popped balloons.
- As confirmed by Todd Kauffman, Jo is based on Sue Sylvester from the TV series, Glee.
- Like Sue Sylvester, Jo rarely calls the other contestants by their names.
- Jo is the first new contestant to be introduced by Chris in the first episode of season four.
- Jo, along with Gwen, are the only contestants who have had their names changed.
- In this case, her name was supposed to be "Mary."
- Jo is the only contestant overall whose voice actor is one of the writers, and in her case, it is Laurie Elliot.
- According to Jo in Backstabbers Ahoy!, she is a champion sprinter, shot-putter, and squat thruster.
- Jo's favorite film is called "Charlie's Devils." This is either a reference to the television show, Charlie's Angels and/or the film.
- Jo is the second newcomer to have conflict with one of the veterans, with the first being Cameron and the third being Lightning.
- Jo is the first new contestant from season four to show nudity, although it is only a silhouette.
- Jo is one of four contestants who has been on both teams in Total Drama: Revenge of the Island, along with Brick, Dakota, and Scott.
- Dakota and Scott were originally on the Toxic Rats while Brick and Jo were originally on the Mutant Maggots.
- Jo is one of only eleven contestants to switch teams, the others being Izzy, Katie, Sierra, Brick, Scott, Dakota, Duncan, Courtney,Cameron, Max, and Sky.
- According to Jo's Total Drama Revenge of the Island contestant biography, her favorite color is gray.
Eliminated from Total Drama: All Stars
in "Evil Dread"
in "Food Fright"